Vaccine therapy and Opsonic method of treatment : a short compendium for general practioners, students, and others / by R.W. Allen.

  • Allen, R. W., 1876-1921.
Date:
1908
Catalogue details

Licence: In copyright

Credit: Vaccine therapy and Opsonic method of treatment : a short compendium for general practioners, students, and others / by R.W. Allen. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    compose a family group of such closely related members as the streptococci. So far as we are aware, there is, for example, but one Bacillus septus and one Micrococcus melitensis ; yet even here the better plan is undoubtedly the preparation of a special vaccine, unless other con- siderations are against the adoption of this plan. The method employed for the isolation of the organisms will vary according as to whether we are dealing with a pure or a mixed infection, and this point may be largely determined by first making smears, staining by Gram's method and with methylene blue, and examining micro- scopically. Should the infection appear to be unmixed, then cultures are to be made at once upon the medium best suited for the growth of the organism in question, such as blood- agar in the case of pneumococcus, nutrose ascitic agar or blood-agar in the case of gonococcus, agar in the case of staphylococcus, glycerine potato in case of tubercle. If the infection be a mixed one, it must be borne in mind as a most important point that the isolation must be done as quickly and in as few subcultures as possible, for only thus is a fully virulent growth likely to be obtained. Of any pecuharities of growth of an organism in pre- sence of others advantage is therefore to be taken, and cultures made from the infected part with every possible care. A few details which have been found useful may here be given. Tubercle Bacillus. The tubercle bacillus is mentioned because the author feels convinced that more and more will it be found advantageous in difficult cases to continue injections I