The microscope and microscopical technology : a textbook for physicians and students / by Heinrich Frey ; translated from the German and edited by George R. Cutter, from the fourth and last German edition.

Date:
1872
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Licence: Public Domain Mark

Credit: The microscope and microscopical technology : a textbook for physicians and students / by Heinrich Frey ; translated from the German and edited by George R. Cutter, from the fourth and last German edition. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    Section Stxtl). THE PREPARATION OF MICROSCOPIC OBJECTS. With the exception of the finished preparations of a collec- tion, in most cases the objects to be examined require prepara- tion, which, as we have already remarked, should be as careful and cleanly as possible. It is only when the blood, mucus, pathological fluids, etc., are examined, that the mere spreading out of a drop of the same is sufficient. The object slides, which are simply strips of glass, serve for the reception of the object to be examined. Several dozen of them should be kept on hand in a clean condition, protected from dust in an accurately closing box. Good slides should be made of pure glass, preferably without any color, and the edges should be ground, for the protection of the instrument. Too great thickness of the glass renders the use of the stronger objec- tives and the cylindrical diaphragms, which then become neces- sary, inconvenient. Therefore, the thickness of the slides should not exceed The most convenient form is that of a long square (3 inches by 1 inch), but when the stage is narrow they should be of a corresponding width. Square slides are less suitable. One should also accustom one's self to place the object to be examined in the centre of the slide. It is rarely examined in the dry condition, but, as a rule, with the addition of some fluid; as, water, gljxerine, etc. This is to be added at the com- mencement of the preparation. One soon learns to judge of the quantity which is necessar3\ The object to be examined, if it is large, and especially if it is thick,—as for example, when one desires to examine a small embryo or an injected specimen of considerable size,—is to be placed with some fluid in a watch-glass under the microscope.