The Pasteur Institute, Kasauli, India: stages in the preparation of the rabies vaccine: straining an emulsion, made using infected rabbit brain, into a bottle. Photograph, ca. 1910.

1910

1 photograph : photoprint ; sheet 21.5 x 18 cm

Preparation of Semple's vaccine (one per cent carbolised). The weighed brain is ground up in enough carbolised saline to make a 2 per cent emulsion. This is then strained through fine gauze as shown. The bottle is then plugged and placed in the dark at 37 degrees C. for 24 hours. At the end of 24 hours it is tested (animal test) and then sufficient saline added to make a one per cent emulsion. Cultures are then taken to ensure absence of contamination

Wellcome Collection 570996i