Experimental manipulation of gene expression / edited by Masayori Inouye.

Date:
1983
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Experimental manipulation of gene expression / edited by Masayori Inouye. Source: Wellcome Collection.

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    Contents V. Expression of Cloned Genes 47 VI. Conclusions 48 References 49 Developments in Streptomyces Cloning Mervyn J. Bibb, Keith F. Chater, and David A. Hopwood I. Introduction 54 II. Vectors 54 III. Use of Tn5 in Relation to Streptomyces DNA 66 IV. Applications of DNA Cloning in Streptomyces 67 V. Concluding Remarks 79 References 80 Vectors for High-Level, Inducible Expression of Cloned Genes in Yeast James R. Broach, Yu-Yang Li, Ling-Chuan Chen Wu, and Makkuni Jayaram I. Introduction 84 II. Materials and Methods 85 III. Results and Discussion 87 IV. Summary 107 Appendix: Plasmid Construction 107 References 115 Genetic Engineering of Plants by Novel Approaches John D. Kemp I. Introduction 119 II. Novel Approaches to Creating Genetic Diversity 121 III. Concluding Remarks 113 References 134 A.SV2, a Plasmid Cloning Vector that Can Be Stably Integrated in Escherichia coli Bruce H. Howard and Max E. Gottesman I. Introduction 138 II. Materials and Methods 138 III. Results 141 IV. Discussion 150 References 152 Construction of Highly Transmissible Mammalian Cloning Vehicles Derived from Murine Retroviruses Richard C. Mulligan I. Introduction 155 II. General Strategy 157