Leukaemia cytogenetics / H. Jackson Woodliff.

  • Woodliff, H. Jackson.
Date:
1971
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Leukaemia cytogenetics / H. Jackson Woodliff. Source: Wellcome Collection.

    101/168 (page 85)
    LYMPHOMAS 85 Studies of bone marrow material have usually been negative and again a normal finding is of no diagnostic significance (Jacobs, Tough and Wright, 1963). In some cases an increase in random aneuploidy may be present but this is unlikely to be helpful diagnostically (Sandberg et ah, 1964c; Hayhoe and Hammouda, 1965; Woodliif, 1969Zj). More marked abnormalities and the presence of an abnormal cell line may be found in some cases, indicating an infiltration of the bone marrow with malignant cells (Tjio et al., 1963; Sandberg et al., 1964c; Fitzgerald and Adams, 1965). Biopsy material from lymph nodes and other lymphoid tissues has revealed an abnormality in the vast majority of cases and this is, of course, significant. Direct examination usually reveals few metaphases and some workers prefer to use short-term cultures. Both methods are more complicated than peripheral blood and bone marrow examination and therefore less suitable for routine diagnostic work. The abnormalities which have been described in lymph nodes and to a lesser extent in cases in which the blood and bone marrow are involved, includes an increased fuzziness of the chromosomes; an increase in the number and more marked appearance of secondary constrictions, an increase in aneuploidy and the presence of an abnormal cell line. The abnormal cell lines may be aneuploid or pseudodiploid with loss or addition of a normal looking chromosome and in many cases the presence of a marker chromosome. All groups appear to be affected but more particular attention has been paid to the G group chromosomes and a deletion of the short arm of the F group chromosomes which has been found in several cases (Ricci et ah, \9в2а and ò; Spriggs and Boddington, 1962; Sandberg et al., 1964c; Fitzgerald and Adams, 1965; Sasaki et al, 1965; Miles et al, 1966; Spiers and Baikie, 1966 and 1968a; Lawler et ah, 1967; Bauke and Schofiiing, 1968; Kajii et ah, 1968). Tetraploid and near tetraploid cells have been frequently recorded. The presence of any of these abnormalities may be taken as presumptive evidence of neoplastic prohferation, although there are few studies of non-malignant lymphadenopathies with which to make a comparison. In some cases of lymphoma normal results have been obtained from lymph nodes and may have been due to sampling only non-malignant cells; however, the possibility that the lymphoma cells themselves have a normal karyotype must also be considered. L.C.