Abel's laboratory handbook of bacteriology / [Rudolf Abel].

  • Abel, Rudolf Velentine Ludwig, 1868-
Date:
[1912?]
Catalogue details

Licence: In copyright

Credit: Abel's laboratory handbook of bacteriology / [Rudolf Abel]. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    (5) Wash in water. Gonococci dark blue, nuclei pale blue, cells pale red. 3. Acco)xling to Giemsa{see p. 172). 4. According to A. Netsser. (1) Stain in warm saturated alcoholic solution of eosin for several minutes. (2) Drain off the eosin with blotting-paper and at once. (3) Pour on to it saturated alcoholic solution of methylene blue minute). (4) Wash in water. Gonococci and cell-nuclei blue. Cell bodies red. 5. According to Pajjpc-nheim. Stain for one minute in a solution of about 2 pen- knife-points of methylene green, about ^ a knife- point of pyronin (Griibler, Leipzig), dissolved in 5c.c. of aq. dest. (The solution should be blue-violet: find by experiment the best mixture.) Wash in water. Gonococci red, nuclei blue. Secretions containing few gonococci (from old cases of gonorrhoea) are spread out on the slide (p. 60) and stained by Gram's method. Gonococci take the counter-stain. Be careful at each washing with water in this process until the preparation has been decolorized in alcohol. 21. Actinomyces. Cultures are to be obtained on the ordinary culture media, especially on glycerine agar. Make many cultures, including anaerobic ones, since some strains grow only in absence of air. Growth of the first generation is usually slow but later becomes more