Abel's laboratory handbook of bacteriology / [Rudolf Abel].

  • Abel, Rudolf Velentine Ludwig, 1868-
Date:
[1912?]
Catalogue details

Licence: In copyright

Credit: Abel's laboratory handbook of bacteriology / [Rudolf Abel]. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    anaerobic plate cultures one can now obtain isolated colonies from which pure cultures may be made. CuUhatiori from eaiih, S;c. In the first place mice or guinea-pigs may be infected subcutaneously. From these animals, dead after a few days with tetanic symptoms, cultures may be prepared from pus at the site of inoculation. The bacillus grows at the room temperature and liquefies gelatine. The bacillus stains with the ordinary aniline dyes, and is positive to Gram's stain. The spores (drum- sticks) may be stained by the methods described on p. 80. In the body, the bacilli are found only at the site of infection. They are motile with peritrichal flagella. 15. Bacillus Botulinus. Motile by means of 4-8 flagella, possesses terminal spores and is Gram-positive. It is an obligatory anaerobe, grows best at 18-25° C, and liquefies gela- tine. Even very small do^es of a broth culture injected subcutaneously into animals quickly produce motor paralyses, dilatation of the pupil, &c., and finally death. The organism is found in decomposed meat, and does not multiply in the body. 16. Bacillus Fyocyaueus. Growth takes place on all the ordinary culture media. The organism is motile and has one flagellum. Typical strains form dark-green pigment (pyocyanin).