Volume 3

Gene expression / Benjamin Lewin.

  • Lewin, Benjamin.
Date:
1974-1977
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Gene expression / Benjamin Lewin. Source: Wellcome Collection.

    27/952 (page 7)
    Treat streptomycin—sensitive cells with DNA carrying streptomycin—resistance 1 wash with DNAase to terminate reaction I incubate to allow expression of str r \ plate on medium containing streptomycin I count number of transformants c cu 0) .Q E D 1er 4 10- 3 1er 2 io -1 concentration of str r DNA (/ig/ml) Figure 1.1: relationship between number of transformants and concentration of transform ing DNA. The protocol used in this experiment is given above the results, which show that the number of transformants increases in proportion to the concentration of transforming DNA up to a plateau level at which recipient bacteria are saturated with transforming DNA. Data of Hotchkiss (1957).