Microarrays & microplates : applications in biomedical sciences / S. Ye & I.N.M. Day (eds.).

Microarrays and microplates

Oxford, UK : BIOS ; New York : Distributed in the U.S.A. by Springer-Verlag, 2003.

ix, 178 pages : illustrations ; 25 cm.

Copy 1 Donor: Wellcome Trust Sanger Institute.

Instrumentation for Genomics and Proteomics / Sarah K. Stephens, Mark R. Truesdale, Julian F. Burke -- History of the microplate -- Microplate-handling instrumentation -- Applications in genomics -- Applications in proteomics -- Arraying of PCR products onto nylon membranes -- Processing colony arrays -- Expression Microarrays / Carl Whatling, Shu Ye, Per Eriksson -- Array fabrication -- Preparation of labelled probes -- Microarray hybridization -- Generation and analysis of expression data -- The need for databases -- Validation of gene expression changes -- PCR amplification of cDNAs -- PCR product purification by precipitation -- Post-processing of glass slides -- Fluorescent probe labelling -- Pre-hybridization -- Microarray hybridization and washing -- Tissue Microarrays: A High-Throughput Technology for Translational Research / Susan Henshall -- Tissue microarray construction -- Staining of tissue microarrays -- Tissue microarray data and image management -- Applications in translational research -- Validation of tissue microarrays -- Future developments -- Protein Chips and Microarrays / C. David O'Connor, Karen Pickard -- Microarray technology -- Versatility of protein microchips -- Single Nucleotide Polymorphism Genotyping Using Microarrays / Tomi Pastinen -- DNA microarrays for sequence analysis -- Arrays for resequencing and SNP-scoring -- Multiplexed allele-specific extension coupled with tag-arrays -- Microplate-Based mRNA Analysis / Taku Murakami, Masato Mitsuhashi -- Microplate-based RNA purification.

Includes bibliographical references and index.