The significance of phosphoric esters in metabolism / by Robert Robinson.

  • Robison, Robert, 1883-1941.
Date:
1932
Catalogue details

Licence: In copyright

Credit: The significance of phosphoric esters in metabolism / by Robert Robinson. Source: Wellcome Collection.

    129/136 page 101
    extensive as in the 9-day culture and there is no definite boundary between epiphysis and diaphysis. Note the large size of the epiphysial regions relative to the shaft. (Mallory’s triple stain.) Fig. 31. Section of a 6-day embryonic femur after 21 days’ cultivation in vitro. The epiphysis is sharply marked off from the diaphysis by the zone of flattened cells; a sheath of bone invests the exten- sive zone of hypertrophied chondroblasts. (Safranine and picro- indigo-carmine.) Fig. 32. Section of a 6-day embryonic femur after 27 days’ cultivation in vitro. (Safranine and picro-indigo-carmine.) Plate VII. Figure 35 (facing p. 93) Fig. 35. Osteogenesis in a hanging-drop culture of mesoderm from a 6-day embryonic jaw. The figures are drawn from camera lucida sketches of a living culture and were made at different stages of growth to show the development of an ossification centre. After 6 days’ cultivation this specimen was fixed and sectioned. A section is shown in Fig. 36. Plate VIII. Figures 36-37 (facing p. 93) Fig. 36. Histological section of culture shown in Fig. 35 after 6 days’ growth in vitro. Fig. 37. Photograph of an 8-day coverslip culture of the soft tissue from a 6-day embryonic jaw. This specimen was stained with silver nitrate and mounted whole. An ossification centre which developed during cultivation, stained black with the silver nitrate indicating that true calcified bone had been formed.
    digitised image 1
    digitised image 2
    digitised image 3