A laboratory guide for histology : laboratory outlines for the study of histology and microscopic anatomy / by Irving Hardesty ... With a chapter on laboratory drawing by Adelebert Watts Lee ... With 30 illustrations 2 of which are in colors.

  • Hardesty, Irving, 1866-
Date:
1908
Catalogue details

Licence: Public Domain Mark

Credit: A laboratory guide for histology : laboratory outlines for the study of histology and microscopic anatomy / by Irving Hardesty ... With a chapter on laboratory drawing by Adelebert Watts Lee ... With 30 illustrations 2 of which are in colors. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

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    relation to other cells ? Note separated muscle fibrillae (sarcostyles). Staining character of the intercellated disks or lines of junction between cells? (b) From a specially stained (Kolossow's method) thin section of heart muscle make a drawing of i or 2 cells showing their shapes, struc- ture, and interrelations. Note carefully the arrangement of the anisotropic substance resulting in the two forms of striation. Cement substance between fibers and cell membranes? Does a branch of one fiber always join a branch of another? Structural characters of the intercellated disks or lines of junction of cells? Fibril bundles, their arrangement in cross-section, and the role of Krause's membrane in them? Consider the peripheral mem- branes, connecting Krause's membranes, along the sides of the fibril bundle, as a sarcolemma and note the effect of such a consid- eration upon the conception of individual cardiac cells. From the studies made of smooth and cardiac muscle, what may be said of evidences that the original syncytium, from which they are derived, is maintained ? 2. Skeletal or ^^voluntary^' muscle. (a) Fresh. Remove a small shred of an extended skeletal muscle of a mammal which has been chloroformed several hours. Tease finely on the slide in a drop of normal salt, cover and examine. What is the color of the tissue? What can you observe as to the shape and appearances of a single muscle fiber (cell)? Now r|move the cover, replace the salt solution with a drop of i per cent acetic acid, let this act 5 minutes, remove it with filter paper, and mount in glycerin. Under high power distinguish the more evident anisotropic elements (fibrils or sarcostyles) surrounded by the isotropic substance (sarcoplasm). Number of nuclei in each fiber? Where are the nuclei situated? Look for demon- strations of the sarcolemma where fibers have been compressed by the needles in teasing, and also for the end of a fiber. Sketch a short segment of a single fiber. What is the sarcolemma ? Com- pare the cell with a syncytium. Measure the diameter of 10 fibers and determine their average thickness in micromillimeters. What length may they attain ? (b) Areas of Cohnheim. For comparison, use stained transverse sections of striated muscle from the human subject, the frog, and an insect (hydrophilus). From each of these (under high power) sketch a'transverse section of a single muscle fiber choosing those with the most apparent grouping of the sarcous elements or fibrilkc (muscle columns) in the sarcoplasm. What is the relation of the components of these areas of Cohnheim to the transverse and