On the fractionation of agglutinins and antitoxin / by Robert Banks Gibson and Katharine R. Collins.

  • Gibson, Robert Banks.
Date:
1907
Catalogue details

Licence: In copyright

Credit: On the fractionation of agglutinins and antitoxin / by Robert Banks Gibson and Katharine R. Collins. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

    17/22 (page 247)
    1:1000) from the typhoid agglutinin in horse serum (1:20,000) was made. The euglobulin was precipitated by adding a half volume of saturated ammonium sulphate solution directly to the mixed sera. The high agglutination values are again given as direct observations, though probably calculated for the original undiluted sera. The figures for the reprecipitated euglobulin are for the typhoid, 1:3000, and for the cholera 1:1600, (an increase over the value of the original goat serum); the pseudo- globulin (3.3 saturation filtrate) agglutinated the typhoid at 1:16,000, the cholera at 1: 20. TABLE X. FRACTIONING OF ANTICHOLERA-ANTITYPHOID HORSE SERUM. Horse 254; bleedings of 11/27/06 and V/29/06; fractioned X/8/06. Fractions tested with the Mt. Sinai culture of typhoid. Centrifuged. Fraction. 100 200 500 1000 2000 2 hrs. after pre- cipitation. .. 11/27/06 Total gbl. Pseudogbl. Eugbl. + + + + + + + + + + + + + + + + + + + +++ + + + + + + + + + + + ± ± + After 12 hrs. Total gbl. Pseudogbl. Eugbl. + + + + + + + + + + + + + + + + + + + +++ + + + + + + + + + + + + ± ± + + + V/29/06. Serum + + + + + + + + + + + + + + + + After 2 hrs. . . . Total gbl. Pseudogbl. Eugbl. + + + + H—1—1—b + 4- + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + ± After 12 hrs... . Total gbl. Pseudogbl. Eugbl. + + + + + + + + ++++ + + + + + + + + + + + + + + + + ± + + + + + + In the following observations it is shown that a relatively large proportion of the typhoid agglutinin may occur in the euglobulin fraction.1 Six cc. of each serum were diluted with 13.8 cc. of water and precipitated by the gradual addition of 10.2 cc. of saturated ammonium sulphate solution. Uniform samples (15 cc.) of each 1 On resuming this problem in the fall of the year, it was found that our cholera culture was spontaneously agglutinating; it could not therefore be employed in testing the agglutination values of the fractions.