On the fractionation of agglutinins and antitoxin / by Robert Banks Gibson and Katharine R. Collins.

  • Gibson, Robert Banks.
Date:
1907
Catalogue details

Licence: In copyright

Credit: On the fractionation of agglutinins and antitoxin / by Robert Banks Gibson and Katharine R. Collins. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    of 1 :iooo, the bulk of the agglutinins was found in the pseudo- globulin (Table IX); the greater portion was also in the high fraction when the serum was precipitated at 3.6 and again at 3.8 saturation. There is no evidence presented here that the precipitation limits of the cholera agglutinin are in any way dif- ferent from that of the typhoid. In the second fractionation (V/10/06) it is seen that both the Pfeiffer and the cholera are increased in the eu-fraction as contrasted with the result of the first precipitation at 3.4 saturation. TABLE VIII. FRACTIONING OF ANTIDYSENTERY HORSE SERUM. Organism. Fraction. 50 100 200 500 1000 2000 Flexner Manila. . .. Serum + + + + + + + + + H—1—b -|—1—1—b + + + + + Total gbl. + + + + + + + + H—1—1—b + + + + + Pseudogbl. + + + + + + + + + + + + + + + — Eugbl. + + + + + + + + + + + ± — — Shiga Serum + + + + + + + + + + + + -|—1—1—b + + + — Total gbl. -b -b + + + + + + + + + + + + + + ± — Pseudogbl. + -b + + -b + + + + + + + — — — Eugbl. + + + + + + + + + + + — — — Colon Serum + -b -b + + + + + + + + + + + + + + + + + 0 Total gbl. + + -b + + -b + + + + + + + — — Pseudobgl. + + + + + + + + + + + + ± — — Eugbl. + + + + + + + + + ± — — The fractionation of the anticholera and antityphoid horse serum is of especial interest because an experiment of this nature is the most striking of E. P. Pick’s observations. Pick had found that the typhoid agglutinin was precipitated with the pseudo- globulin fraction in horse serum; the cholera agglutinin, on the contrary, came down with the euglobulin. Pick, therefore, mixed equal volumes of a typhoid and a cholera serum, and pro- gressively precipitated 2 cc. amounts of the mixed sera at 2.8, 3.0, 3.2, etc., saturation (with a final volume of 10 cc. in each case). A well marked separation of the cholera agglutinin into the euglobulin and the typhoid agglutinin into the high fraction resulted. As given in Pick’s tables, the observed agglutination values are twice too much, since each serum was diluted a half by the mixing. A direct separation at 3.4 saturation of the agglutinin in a goat cholera serum (agglutinating at a dilution of