Linkage maps of human chromosomes / Howard Hughes Medical Institute.

Date:
1987
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Linkage maps of human chromosomes / Howard Hughes Medical Institute. Source: Wellcome Collection.

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    AN AUTOMATED SYSTEM OF LINKAGE ANAIATSIS (Gene Mapping System or GMS) G.M. Lathrc^, P.Cartwriqht. J.M. Lalouel Howeird Hughes Mediceli Institute, University of Utah, Seilt Lake City, USA. We have developed an autcraated system of linkage 2in2ilysis, GMS, to aid in the exinstruction of humein genetic maps. ■Ше GMS program interfaces with LINKAGE, cind uses a databeise of genotypes on 59 reference femiilies, including 38 families fron the СЕШ panel. Genetic maps are constructed fron markers eissigned to each chrcgnosame using a series of algorithms to select apprc^riate multilocus tests. Hie strategy enployed in GMS is to use two-point led scores to construct a preliminary map (tried map). Multilocus einalysis of the trial map gene order results in the division of the loci into sub-linkage groups of closely linked mcirkers; gene orders are determined within each group sepeurately, and the order and orientations of the groups are established by multilocus analysis. A variety of different eilgorithms have been inplemented for the selection of the appropriate tests vAien the number of sub-linkage groups excludes the possibility of testing all orders eind orientations. Validation runs eire made using different gene orders within the sub-linkage groups, and different initial divisions of the loci. When the maximum likelihood gene order is not the scime cis the triad map, the former is used to establish new sub-linkage grotçs, eind the einadysis is repeated. The GMS program h2is been used to establish primairy maps of nost chrcTODsames (see eibstracts on chrcmasones 1, 6, 7, 9, 11, 12, 13, 14, 15, 16, 17, 18, emd 19) . Recoibination rates eind likelihoods frcm all tests are stored in a results databeise. This database will permit rapid evaduation of other algorithms for obtaining triad maps eind for restricting tests of edtematives when seeirching for the maxijnura liJcelihood gene order. 13