The microscope and microscopical technology : a text-book for physicians and students / by Heinrich Frey ; translated and edited by George R. Cutter.

Date:
1880
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Licence: Public Domain Mark

Credit: The microscope and microscopical technology : a text-book for physicians and students / by Heinrich Frey ; translated and edited by George R. Cutter. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    Ebner, this is very scanty and soft, so that these canals may regularly fall apart. The organ of such creatures, hardened in Muller’s fluid and alcohol, requires, therefore, a preliminary imbedding (p. 113) when fine sections are to be obtained. In other animals, for example, the calf (Frey), the cat, and the boar (Mihalkovics), the latter procedure may be dispensed with. We would rec- ommend hsematoxyline as the best coloring material. For the isolation of the seminiferous canals, employ the maceration in muriatic acid (p. 125). Mihalkovics recommends for the human testicle, the immersion in f hydrochloric acid and water, allowing the action to continue for a day or two at a temperature of about 30° C. and subsequent removal to dis- tilled water, till the preparation comes apart. The remainder is completed with the prepar- ing needles. We have already (p. 280) mentioned the coarsely granulated, so-called plasma-cells of the connective tissue (fig. 351) which be- sides occurring in other organs is also pro- fusely developed in the testicles of many mammals as an investing substance, around the finer blood-vessels. We recommend the rat and the cat for their study, and hseinatoxyline as a color- ing medium. The preliminary puncturing method and the in- jection of weak (0.25 per cent.) solutions of osmic acid, with subsequent hardening in absolute alcohol, affords excellent views of these cells, as well as of the genesis of the seminal filaments which is to be discussed hereafter. The seminal canals then coalesce and finally form a single vessel, a tolerably large canal, which, with innumerable convo- lutions, forms the so-called body and tail of the epididymis; it afterwards straightens and becomes the vas deferens. The epididymis shows, in addition, a ciliated lining of its convo- luted seminiferous canals (Becker), in places with gigantic cells and vibratile cilise. The blood-vessels, which are very easy to inject, pass from without and from the corpus Highmori into the organ, per- meate the septula, and finally encircle the seminiferous canals with a wide-meshed (but not particularly abundant) net-work of capillaries. Fig. 351. So-called plasma- cells, 6, surrounding a blood- vessel, a, of the rat’s testicle.