"The three-dimensional Patterson function of ribonuclease"

Date:
[1956]
Reference:
PP/CRI/H/2/9
Catalogue details

Licence: In copyright

Credit: "The three-dimensional Patterson function of ribonuclease". Source: Wellcome Collection.

    2/13
    eeüe available (king, Kagdoff, Adelman and Marker, 195- ) Is ribo- iplLi*. ijrïu-p nuclease II ( P 2 1 , one molecule per asymmetric unit) and this also * A haß the advantage■■of being thè fora post easily*.obtainable* . It was . decided therefore to collect the intensities e£ ribonuclease II out S«*! K * * -f^e/Krw to 2 = 40° and to oonspute a three-dimensional Patterson from them* a * It is understood that Carlisle and his co-workers have iIbo calculat- ^ JpA.r\ch**y ¡,j~ ed a slasil ar Patterson, but at the tira e of writing t-iiM has not been published, though sections from it have been shown at various meetings# Th* tLAA* Wo*s lAhjpy vv\ U.S Kv«* a C. Hour w. ft» c Cívu^t »i fr*c «* cK¿Vs Xrtr f a.\> £¿ e.«✓+ 'wv Experimental re clinique . J The methods frf> growing eibonuclease crystals have been describ ed elsewhere (King, agdoff, Adelrean and Marker, 195- ) * These chosen 1^*« i-ii io-tvc were grown from $0% tertiary butyl alcohol* oaly .two—erystale—were WVI KV\.é^ +M>Ç -^»94=^0 •U'jlltjc4, -th» Intent it teja». .fhe fr/Ist aas used for 2 = 0° to ^ Xfe * lO j lo ¡fu ÍUn OtCKÍ tsTnjJ! )>\Í.*.Í tVJ-ftKftvd and the neoond for 2 5 30® to 40°, plus .go.me—ef-the firot cot »to enable both sets to be put on t-e same scale* The experimental data vier e collected by two of ue and V*L.) with the helo of all our coleauea. The Intensities were mea- o^On^cMj) aured on a General nei gor» nAimt.^ n ^ t mod if led for sinjâ