Find thousands of books, manuscripts, visual materials and unpublished archives from our collections, many of them with free online access.

Hormone Assays in Biological Fluids / edited by Michael J. Wheeler, J. S. Morley Hutchinson.

Wheeler, M. J.
Date
2006
  • Books
  • Online


About this work

Description

Although automated systems can measure all common hormones in human blood, they are expensive and often incapable of measuring hormones in urine, saliva, and animals. In Hormone Assays in Biological Fluids, expert researchers who have developed and applied significant new assays describe in step-by-step detail readily reproducible methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can develop novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and polymerase chain reaction techniques help readers choose the best methods for their purpose. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and highly practical, Hormone Assays in Biological Fluids illuminates the many different methods available for a broad range of hormone assays, enabling researchers to choose the optimal existing methods or develop novel assays for their specialized purposes.

Publication/Creation

Totowa, NJ : Humana Press, 2006.

Physical description

IX, 258 pages : online resource.

Type/Technique

Languages

  • English


Permanent link


Identifiers

ISBN

  • 9781592599868

We’re improving the information on this page. Find out more.