The blood : a guide to its examination and to the diagnosis and treatment of its diseases / by G. Lovell Gulland and Alexander Goodall.

  • Gulland, G. Lovell (George Lovell), 1862-1941.
Date:
1912
Catalogue details

Licence: Public Domain Mark

Credit: The blood : a guide to its examination and to the diagnosis and treatment of its diseases / by G. Lovell Gulland and Alexander Goodall. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

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    2. Gowers' solution— Sodium sulphate Acetic acid . Distilled water 3. Hay em's solution— ' Perchloride of mercury Sodium sulphate Sodium chloride Distilled water 104 grs. 1 drm. 6 ozs. 05 grm. 5 grms. 1 grm. 200 grms. This solution is deservedly the one most commonly used. It pre- serves well both the shape and colour of the red corpuscles. It keeps well, though a deposit forms after a time. This can be filtered off without spoiling the solution. 4. Toisson's solution— Sodium sulphate 8 grms. Sodium chloride 1 grm. Methyl violet 5 B . 0-025 grm Glycerine (neut.) . 30 c.c. Distilled water . 160 c.c. j^i ^ 1 i n -1 _ This solution stains the nuclei of the leucocytes while conserving the red cells. Some of the latter, however, when pale, are not well seen, and the count is therefore apt to be too low. The methyl violet sometimes precipitates, and there is no great practical advantage in the staining of the leucocyte nuclei. The Thoma-Zeiss Hcemocytometer.—This instrument is the one in common use, and is the most convenient. It is designed to indicate the number of corpuscles in a cubic millimetre of blood. As supplied it consists of two pipettes and a counting chamber. One pipette has a fine bore and a large bulb by which a sample of blood may be diluted either 100 or 200 times, the other has a wide bore and a small bulb by means of which blood may be diluted either ten or twenty times. The latter is specially intended for the enumeration of white cells. In the case of the red cell pipette, if blood be taken up to the mark 0'5, and diluting fluid up to the mark 101, the dilution will be 1 in 200; if blood be taken up to the mark 1, and diluting fluid to the mark 101, the dilution will be 1 in 100. The most convenient dilution is 1 in 200, unless the blood be very poor in corpuscles, when 1 in 100 should be used. Procedure.—The diluting fluid to be used should be at hand in a bottle, or a little may be poured out into a watch-glass. The ear