Bacteriological techniques for dairy purposes.

  • Great Britain. Ministry of Agriculture, Fisheries and Food.
Date:
1968
Catalogue details

Licence: Public Domain Mark

Credit: Bacteriological techniques for dairy purposes. Source: Wellcome Collection.

    29/192 (page 21)
    10. MEDIUM FOR TOTAL SPORES 1. Starch Milk Agar Soluble starch : : é Ig Peptone , : , I0og Lab. lemco . : Iog Sodium chloride. ae | See , 5g Separated milk Ash Bite ek OTL ASars sy : ; ; 20 g Distilled “ay : ’ q 1,000 ml Dissolve the starch, peptone, lemco, sodium chloride and agar in the distilled water, filter and adjust pH to 7-0. Add the separated milk. Distribute 10 ml quantities in 6 < 3? in. test-tubes and autoclave at 121° for 15 min. See Ref. 1. Note If New Zealand agar is used, 15 g will suffice. REFERENCE 1. GRINSTED, E. and CieGG, L. F. L. (1955). #. Dairy Res., 22, 178. 11. MEDIA FOR ANAEROBIC SPORES 1. Reinforced Clostridial Medium (R.C.M.) Yeast extract (Yeastrel) . : 3g Peptone (Evans) . 10g Sodium acetate A.R. (anhydrous) 5g Cysteine hydrochloride . ; wRUno Ws tg Soluble starch : ‘ j Ig Agari : f : O-5g Distilled Sie ‘ : ; 1,000 ml Steam to dissolve all ingredients. Add 10 g of dextrose and dissolve by - shaking. Adjust the pH to 7:2. Add aqueous neutral red solution to give a final concentration of 1/10,000 and autoclave at 121° for 15 min. 2. Clostridial Iron Sulphite Medium (a) Basal Medium: Tryptone ; ' ; : Is g Yeast extract powder— moisture less than § per cent 10g Agar. : j ; Is g Distilled lee ; f 1,000 ml Adjust pH to 7:0--0-1 with 10 per cent caustic soda. Sterilize for 20 min. at I21° (b) Sodiven Sulphite Solution: Sodium sulphite (SO, content about 23 per cent) : Iog Distilled water . . roo ml Sterilize by filtration through a G§5 or Hs glass filter,