Splicing life : a report on the social and ethical issues of genetic engineering with human beings / President's Commission for the Study of Ethical Problems in Medicine and Biomedical and Behavioral Research.

  • United States. President's Commission for the Study of Ethical Problems in Medicine and Biomedical and Behavioral Research.
Date:
1982
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Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Splicing life : a report on the social and ethical issues of genetic engineering with human beings / President's Commission for the Study of Ethical Problems in Medicine and Biomedical and Behavioral Research. Source: Wellcome Collection.

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    34 Splicing Life: Chapter 2 Figure 6: Splicing Human Gene into Plasmid E. coli bacteria, taken from human intestine Pancreas Nucleus Plasmid E. coli chromosome I Plasmid removed from E. coli Plasmid cut open by restriction enzyme at a specific site Human cell Strand of DNA from human cell Human DNA cut I . into pieces W by restriction enzyme Two pieces spliced together Recombinant DNA (hybrid plasmid) Human insulin gene Hybrid plasmid inserted into E. coli cell Source; President's Commission. Bacteria with hybrid plasmid replicate, creating clone capable of producing insulin