Exercises in practical physiology / by Augustus D. Waller and W. Legge Symes.

  • Augustus Desiré Waller
Date:
1897
Catalogue details

Licence: Public Domain Mark

Credit: Exercises in practical physiology / by Augustus D. Waller and W. Legge Symes. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

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    Directions. _ Result. Native albumins [solutions of egg albumin, and of serum albumin free from globulins). 7 Faintly acidify and boil 8 Saturate witli sulphate of magnesium . 9 Acidify, add one volume of ether, and shake The albumin is coagulated. The albumin is not pre- cipitated. (' Egg albumin is , usually I coagulated. Serum albumin is not usually coagulated. Globulins [solution of serum globulin, or of muscle globulin). 10 Faintly acidify, and boil .. .. .. The globulin is coagulated. 11 Saturate with sulphate of magnesium .. The globulin is precipi- tated. 12 Let a few drops fall into distilled water .. A cloud, i.e., a faint precipi- tate of globulin appears. Derived albumins [prepa^-ed by treating ncotive albumins, or globulins ivith dilute acid or alkali). 13 Take reaction U Boil 15 Cautiously neutralise, shaking after each addition of neutralising re-agent, then add slight excess of the neutralising re-agent Albumoses— 16 Faintly acidify, and boil 17 Apply biuret test 18 Saturate with sulphate of magnesium .. 19 Cautiously add nitric acid, or salicyl-srd- phonic acid, and shake .. then heat the test tube and then cool the test tube under the tap Peptones— 20 Faintly acidify, and boil 21 Apply the biuret test 22 Add nitric acid It is acid, or alkaline, not neutral. Derived albumins are not coagulated. Derived albumins are pre- cipitated. The precipitate re-dis- solves. Albumoses are not coagu- lated. A pink coloar results. Some albumoses (primary albumoses) are precipita- ted. A precipitate forms. The precipitate dissolves. The precipitate re-appears. Peptones are not coagu- lated. A pink colour results. Peptones are not precipi- tated.
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