Movie of necrotic cell death

Movie of cell death, made using a fluorescence microscope. This field of cells has been exposed to highly toxic moderately oxidised LDL. The cells are predominantly dying by necrosis. All cells are stained with the cell-permeable blue hoechst dye, which stains nuclei (DNA). Phosphatidylserine rearrangement is detected with annexin-V-fitc (green). Necrotic cells stain red as the plasma membrane is compromised and becomes permeable, taking up propidium iodide. They stain green significantly later than in apoptosis as the annexin-V-fitc slowly diffuses through the cytoplasm. Compare B0005598, movie of cell death by apoptosis using the same stains.