Dental anatomy note book ... / by Douglas Gabell.

  • Gabell, Douglas Phillimore.
Date:
1909
Catalogue details

Licence: In copyright

Credit: Dental anatomy note book ... / by Douglas Gabell. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    28 COLLODION IMBEDDING (for larf=;e objects). 1. Dehydrate in absolute alcobol. 2. Soak in a mixture of equal parts of alcohol and ether. 3. Place in a very thin solution of collodion. 4. Place in a thick 5. Allow the solution to evaporate slowly. 6. Eemove the object to 30 % alcohol to harden. 7. Cut with a microtome. H. Stain and dehydrate. 9. Clear in cedar oil (not oil of cloves). 10. Mount. The time taken will depend on the size and permeability of the object. It is better, when possiiile, to make cuts in the specimen to hasten penetration. PARAFFIN IMBEDDING (for small objects and very thin sections). 1. Dehydrate.- 2. Clear in cedar oil. 3. Place in melted paraffin (45° C.) still satui'ated (1 hr.). 4. Cool rapidly. (To prevent crystallisation.) 5. Mount on a microtome and cut. 6. Warm and wash out paraffin with naphtha. > 7. Stain, clear, mount. In either method the object may be stained in mass ])efore imbedding, if preferred. STAINS. ALCOHOLIC BORAX CARMINE (for stainino in bulk). 1. Place in the stain till saturated 2—4 weeks. 2. Place in acid alcohol to fix the stain 12 hours. 3. Dehydrate in 90 % and 100% neutral alcohol . 12 hours each. ACID ALCOHOL:-70% ALCOHOL AND 2 DROPS HCl TO A TEST TUBE FULL. SILVER NITRATE. 1. Wash the fresh tissues in distilled water. 2. Place in 1 % AgNOg in the sunlight, till of a whitish-grey colon}-. 3. Wash and mount at once.