Blood chemistry, colorimetric methods for the general practitioner : with clinical comments and dietary suggestions / by Willard J. Stone.

  • Stone, Willard J. (Willard John), 1877-1943.
Date:
1926
Catalogue details

Licence: In copyright

Credit: Blood chemistry, colorimetric methods for the general practitioner : with clinical comments and dietary suggestions / by Willard J. Stone. Source: Wellcome Collection.

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    10. The blood pipettes and blood-sugar tubes of Folin, with bulb and constriction, as well as Pyrex ignition test-tubes, can be obtained from any dealer in scientific supplies. 11. The choice of the colorimeter will depend upon the individual. The Duboscq is now made in this country, as well as the Kober and the Klett model. Formula Jor Calculation in Colorimeter Work The following formula serves as the basis for computations in which different quantities of unknown and varying strengths of standard solutions are used: Standard Strength of Reading ^ Standard in Mg. Unknown Volume of Standard as Reading Compared with Unknown Mg. in Amount of Blood or Urine Used. The Normal Blood Constituents of Clinical Importance Per 100 c.c. of Blood Urea nitrogen 12.0 - 15.0 mg. Uric acid 1-5 - 4-5 mg. Preformed creatinin 1-5 - 2.0 mg. Total creatinin (creatin plus creatinin) 4.0 - 6.0 mg. Amino-acid nitrogen 6.0 - 8.0 mg. Ammonia nitrogen 0.1 - 0.2 mg. Total non-protein nitrogen (the incoagu- lable nitrogen)1 25.0 - 35.0 mg. Sugar 80.0 - 110.0 mg. Cholesterol 160.0 - 200.0 mg. Chlorides (as NaCI) 600.0 - 650.0 mg. 1 The term non-protein nitrogen includes those nitrogenous substances remaining after precipitation of the proteins.