The inhibition of lactic acid formation in cancer and muscle / by Sylvia Thurlow Harrison and Edward Mellanby.

  • Harrison, Sylvia Thurlow.
Date:
[1930?]
Catalogue details

Licence: In copyright

Credit: The inhibition of lactic acid formation in cancer and muscle / by Sylvia Thurlow Harrison and Edward Mellanby. Source: Wellcome Collection.

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    Since Case and McCullagli liad shown the inhibiting factor for muscle glycolysis to be present in other extracts containing amylase, we tried the effect of such extracts on the aerobic glycolysis of cancer tissue. Malt diastase and takadiastase extracts were made by extracting 0*5 g. dried commercial preparation with 5 cc. water for 1 hour, filtering and adjusting to about pH 7*6. The salivary gland preparation was made as follows. The submaxillary gland of an ox was minced, ground with acetone twice, filtered, ground with ether twice, filtered and dried in air. 0-2 g. was extracted in 8 cc. water. The effects of malt diastase, takadiastase and salivary gland extracts on the aerobic glycolysis of cancer tissue are shown in Table II. Table II shows that in 22 experiments in which malt diastase, taka¬ diastase or dried salivary gland was used, there were 19 cases in which the presence of one or other of these preparations caused an inhibition in the aerobic glycolysis of cancer tissue. Similar experiments using pancreatic extracts were then made anaerobic¬ ally. The experiments were carried out in the manner already described except that the gas mixture used for filling the flasks consisted of 95 % nitrogen and 5 % C02. The results are given in Table III. The pancreatic extract used in these experiments was prepared according to the method of Ronzoni, Glaser and Barr. The tissue used in all the experiments in this table was mouse carcinoma 63. Table III. No. of Pancreatic extract mm.3 C02 with Inhibition exp. cc. glucose o/ /o 1 0-1 heated 29-5 0-1 211 28 2 0-1 heated 111 0-1 1-2 89 3 0-1 heated 15-8 Ol 3-3 79 4 0-1 heated 6*5 01 10 84 5 0-1 heated 240 0-1 13-0 46 6 0-1 heated 10-2 01 7*5 26 7 0-1 heated 11-7 0-1 0-4 96 It can be seen from Table III that pancreatic extract made according to Ronzoni, Glaser and Barr’s directions caused an inhibition (ranging from 26 to 96 %) in the anaerobic glycolysis of cancer tissue in all of the seven experiments. Several experiments were then carried out on a larger scale, in which the lactic acid formed was estimated directly by the chemical method of Friede- mann, Cotonio and Shaffer [1927]. The pancreatic extracts for these experi¬ ments were made according to Ronzoni, Glaser and Barr, a number of different preparations being used. A control was always done using Ringer