BUdR

Date:
1970
Reference:
PP/CRI/H/4/18
Catalogue details

Licence: In copyright

Credit: BUdR. Source: Wellcome Collection.

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    sequent generations in thymidine! Theory predicts that the result w ill be (Thymidine) BUdR Thymidine generation 0 12 3 fraction of cells working. effectively mononucleate 1 0 \ I vt' etc. haploid binucleate - 1 (1) (1) brackets, because these cells will have fused and therefore ceased dividing. We can also ask the following question: Suppose we grow cells for two generations in BUdR, and make them binucleate at the second generation, how many should work? The answer is none.' This should be contrasted with the monon ucleate case above where there was one generation in BUdR and then a second one in thymidine, after which half should work. Unfortunately, it takes a little time in the myoblast case to show that a cell has started to work, i. e. produce muscle protein. It can only be estimated by the delay produced by the BUdR (preferably expressed as number of divisions rather than time). But another system might be scorable at an earlier time. 7. If the activity (after one generation in BUdR and then subsequent generations in thymidine) returns more slowly than predicted (as seems to be rather likely)