The microscope and microscopical methods / by Simon Henry Gage...and illustrated by 165 text figures in the text.

  • Gage, Simon Henry, 1851-1944.
Date:
1896
Catalogue details

Licence: Public Domain Mark

Credit: The microscope and microscopical methods / by Simon Henry Gage...and illustrated by 165 text figures in the text. Source: Wellcome Collection.

Provider: This material has been provided by the Harvey Cushing/John Hay Whitney Medical Library at Yale University, through the Medical Heritage Library. The original may be consulted at the Harvey Cushing/John Hay Whitney Medical Library at Yale University.

    187/270 (page 167)
    § 278. Staining the Sections with an Alcoholic Dye.—With an alcoholic stain like hydrochloric acid carmine, remove the slide from the alcohol and add the stain directly after draining the slide. Do not allow the slide to become dry, for that would probably ruin the tissue. Wash away the stain with 67 '/< alcohol then dehydrate with 95'/( alco- hol and clear and mount in balsam as described below. § 279. Staining with an Aqueous Dye.—Wash away the alcohol by plunging the slide bearing the sections into a jar of water and mov- ing it around a moment. Then add the stain to the sections with a pipette and allow the slide to rest horizontally on the bars of the waste bowl (Fig. 134) or preferably immerse the slide in a jar of the stain ($ 264). The sections are usually well stained in from 1 to 10 min- utes when they should be thoroughly washed with water. § 280. Staining with a General Dye.—If it is desired to give a general stain after the nuclear dye, carmine stained preparations can be stained half a minute or more with picric-alcohol (§ 315) and the hem- atoxylin stained specimens with eosin (§ 305J. It usually takes only 10 to 20 seconds for this. Then wash away the stain with 67^ alcohol. S 2S1. Dehydration of the Stained Sections.—Place the slide with the stained sections in a jar of 95% alcohol for a few minutes or wave it around for a minute or two and the preparation will be ready for clearing and mounting. S 282. Clearing the Sections.—Drain off the alcohol and add a drop or two of clearer (Carbol-turpentine (S 303). If any whiteness or cloudiness remains the sections were not sufficiently dehydrated. Complete the dehydration by washing away the clearer with 95'/ alco- hol and soaking the slide a few minutes in a vessel of the same. § 283. Mounting in Balsam.—For this the clearer is drained away by standing the slide nearly vertically on some blotting paper or by putting it in the funnel of the waste bowl. The balsam is then placed on the sections and the cover-glass added or the balsam is spread on the cover-glass. For labeling and cataloging and storing the preparations see § 291- 295-