The pathology of malignant disease of the cervix uteri : with a note as to treatment / by John de Ville Mather.

  • Mather, John de Ville.
Date:
1906
Catalogue details

Licence: In copyright

Credit: The pathology of malignant disease of the cervix uteri : with a note as to treatment / by John de Ville Mather. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    alcohol, after which, a solution of alcoholic eosin is placed on the section. When the specimen is stained apparently suffi- ciently, the surplus eosin removed by alcohol, the section is now held over some ammonia vapour. The result is that the excess of eosin at once comes out, the hasmatoxylin is im- mediately brought back into full staining vigour, and one has a section which one can govern, bringing up either nuclei or protoplasm at will. The section is cleared and mounted in the usual way. Another method I used in almost all cases was the acid picrin f uchsin method of Unna, as described in Walker Hall and Herxheimer. I found the method exact and useful, and some of my photographs are taken of slides made by that method. Water-blue and orcein, polychrome methylene blue and orcein, Van Gieson and hematoxylin, and saffranin— these combinations I also used occasionally, but the bulk of the staining was by the hasmatoxylin eosin, and the acid picrin fuchsin. The curettings, of which I only examined a few—between fifteen and twenty at most,—were put immediately into absolute alcohol, instead of formalin or mercuric chloride. Otherwise they were treated in the same way. It was my practice to clear in clove oil, and mount in xylol balsam. The methods of tracing patients was necessarily complicated. In most cases I wrote, or saw their private practitioner. The difficulties arising are great, for many have doubtless died whom I have entered as lost sight of; but, without definite proof of this fact, I have not counted them as dead. I might add that cervix uteri is a singularly difficult tissue to cut, and presents difficulties not present m many of the other tissues of the body.