Working with animal chromosomes / Herbert C. Macgregor and Jennifer M. Varley ; chapter 7 contributed by Aimee Bakken, R. Stephen Hill.

  • Macgregor, Herbert C.
Date:
[1983], ©1983
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Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Working with animal chromosomes / Herbert C. Macgregor and Jennifer M. Varley ; chapter 7 contributed by Aimee Bakken, R. Stephen Hill. Source: Wellcome Collection.

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    Contents xi CHAPTER 9 AUTORADIOGRAPHY 213 I. Autoradiographic emulsions 215 II. Darkroom 218 III. The dipping chamber 219 IV. SHde storage boxes for autoradiographs 219 V. Subbed shdes 219 VI. Checklist of equipment and protocol for preparing autoradiographs 220 VII. Exposure 221 VIII. Development 222 IX. Autoradiography with stripping plates 223 X. Removal of autoradiographic background with peroxide 224 XI. Staining of autoradiographs 225 Additional sources of information 226 CHAPTER 10 MEASURING NUCLEAR OR CHROMOSOMAL DNA 227 I. Microdensitometry 228 II. The Feulgen reaction 233 A. Fixation 233 B. Hydrolysis 233 C. Staining 235 D. Washing 235 E. Dehydration and mounting 236 F. Measuring 236 G. Calibrating 237 References 238 Appendix 1 Names, addresses, and telephone numbers of suppliers 241 Index 245