Quantitative laws in biological chemistry / by Svante Arrhenius.

  • Arrhenius, Svante, 1859-1927.
Date:
1915
Catalogue details

Licence: In copyright

Credit: Quantitative laws in biological chemistry / by Svante Arrhenius. Source: Wellcome Collection.

    81/184 (page 65)
    in a given time, e.g. io minutes. If, for instance, we find that the fourfold quantity is necessary for reaching the same haemolytic effect at 290 C. as at 39° C., we may say that—according to the y/-rule— the same quantity of ammonia would occupy a time four times as long to produce the same effect at 29° C. as at 390 C. Such determinations have been carried out on a very large scale with different haemolytic agents by Madsen and his co-workers Walbum and Noguchi. As an instance, I give a series for ammonia with a time of action of 10 minutes, t is the temperature, q the necessary quantity in cc. of a 0-5 normal NH3 solution. The total quantity was 8 cc. containing 1 per cent of red blood-corpuscles from a horse. yobs is the observed, ^calC) a calculated quantity evaluated by means of the general formula for the influence of temperature on the velocity of reactions. The degree of haemolysis was 17 per cent. Haemolysis by means of Ammonia at different Temperatures t. ^obs. Q calc. 2 1-0 060 064 25-9 0-30 0-30 29.7 o-17 0 17 34-8 0 6 co Ul 0-083 39-5 0-04 0-043 The value of /jl used for the calculation, which agrees very well with the observation, is 26,760. F