Influence of excess vitamin A on the suplhate metabolism of bone rudiments grown in vitro / by H.B. Fell, E. Mellanby, and S.R. Pelc.

  • Fell, Honor B.
Date:
[1956?]
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Licence: In copyright

Credit: Influence of excess vitamin A on the suplhate metabolism of bone rudiments grown in vitro / by H.B. Fell, E. Mellanby, and S.R. Pelc. Source: Wellcome Collection.

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    10. It is tentatively suggested that, under the influence of vitamin A, the cartilage cells may produce an enzyme which results in the formation of a soluble sulphated mucopolysaccharide, instead of the normal chondroitin sulphate, and in the dissolution of existing matrix. The authors wish to thank Lady Mellanby and Dr G. Popjak for helpful and constructive criticism of the manuscript; Mr R. J. C. Stewart, who made the cultures and contributed in many other ways to this work, for his skilful and enthusiastic help; and Mr V. C. Norfield, who printed the photographs and prepared the plates. REFERENCES Amprino, R. (1954). Differenziazione e accrescimento della cartilagine di abbozzi scheletrici studiati con l’impiego del radiosolfo. R.C. Accad. Lincei, Ser. 8, 16, 781-785. Doniach, I. & Pelc, S. R. (1950). Autoradiograph technique. Brit. J. Radiol. 23, 184-192. Dziewiatkowsky, D. D. (1954). Vitamin A and endochondral ossification in the rat as indicated by the use of Sulfur-35 and Phosphorus-32. J. exp. Med. 100, 10-24. Fell, H. B. & Mellanby, E. (1952). The effect of hypervitaminosis A on embryonic limb-bones cultivated in vitro. J. Physiol. 116, 320-349. Fell, H. B. & Mellanby, E. (1953). Metaplasia produced in cultures of chick ectoderm by high vitamin A. J. Physiol. 119, 470-488. Fell, H. B., Mellanby, E. & Pelc, S. R. (1954). Influence of excess vitamin A on the sulphate metabolism of chick ectoderm grown in vitro. Brit. med. J. ii, 611-612. Fell, H. B. & Robison, R. (1929). The growth, development and phosphatase activity of embryo¬ nic avian femora and limb-buds cultivated in vitro. Biochem. J. 23, 767-784. Herbertson, M. A. (1955). The reversibility of the effect of hypervitaminosis A on embryonic limb bones cultivated in vitro. J. Embryol. exp. Morph. 3, 355-365. Lajtha, L. G., Ellis, F. & Oliver, R. (1953). Isotope uptake of individual cells: uptake of S35 sulphate by human bone-marrow cells in vitro. Brit. J. Cancer, 7, 401-406. Pelc, S. R. & Glucksmann, A. (1955). Sulphate metabolism in the cartilage of the trachea, pinna and xiphoid process of the adult mouse as indicated by autoradiographs. Exp. Cell. Res. 8, 336-344. EXPLANATION OF PLATES ep epiphysis ohc old hypertrophic cartilage he hypertrophic cartilage yhc young hypertrophic cartilage me marrow cavity zfc zone of flattened cells Plate 1 Photographs of limb bones of normal embryos, from eggs into which Na235S04 had been injected (in vivo experiment). Figures 3-5 are all to the same magnification. Fig. 1. Hypertrophic cartilage from the femur of a 9-day embryo, fixed 4 hr after injection of Na235S04. The ARG is mainly concentrated above the cells; slide exposed for 7 days, stained with toluidine blue. Fig. 2. Hypertrophic cartilage from the femur of a 9-day embryo, fixed 24 hr after injection of Na235S04. The ARG is now profuse over the matrix as well as over the cells; slide exposed for 7 days, stained with toluidine blue. Fig. 3. Humerus from a 7 day embryo, 4 hr after injection-of Na235S04. The ARG is weak above the hypertrophic cells in the centre of the shaft; elsewhere the incorporation of the 35S04 is fairly uniform, (a), slide exposed for 7 days, stained with toluidine blue; (b) 21 days’ exposure, unstained. Fig. 4. Humerus from a 9-day embryo, 4 hr after injection of Na235S04. The uptake of 35S04 is now confined to the distal parts of the bone, except for a narrow zone of ARG (marked with an
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