Practical histology and pathology / by Heneage Gibbes.

  • Gibbes, Heneage, -1912.
Date:
1880
Catalogue details

Licence: Public Domain Mark

Credit: Practical histology and pathology / by Heneage Gibbes. Source: Wellcome Collection.

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    Let the material remain in this for from 24—36 hours, never longer than three days, and then replace it by pure methylated spirit, it may remain in this for an in- definite time, but it will often be found that the spirit becomes cloudy and full of deposits in a few days; in this case it is only necessary to change the spirit until it remains clear. A large quantity of ± per cent, solution of chromic acid should be kept on hand, and it should be mixed with the spirit as required, it will be found the most useful of all the hardening agents, if it is changed at the proper times, and it should not be used stronger than is given above, it will even be found beneficial to use it weaker in some cases. 2. Muller's Fluid is a good hardening mixture, but requires a much longer time, taking weeks to do what the chromic acid mixture will do in days. It is made thus:— Take Potass. Bichrom. 2 parts. Sodae Sulph. 1 part. Water 100 parts. The advantage of this mixture is that larger pieces can he hardened in it, and it does not require changing after the first week or two, hut it will take from 5—7 weeks to harden anything according to its size. The material, when sufficiently hardened, should be well washed and then placed in dilute spirit in the same manner as recommended after hardening in the chromic acid mixture.