The constituents of the flowers of Trifolium incarnatum / by Harold Rogerson.

  • Rogerson, Harold.
Date:
[1910?]
Catalogue details

Licence: In copyright

Credit: The constituents of the flowers of Trifolium incarnatum / by Harold Rogerson. Source: Wellcome Collection.

    11/16 (page 1012)
    The residue thus obtained was heated with an alcoholic solution of potassium hydroxide in order to remove unchanged phthalic anhydride, when, on cooling, a small amount of a substance was deposited. This was collected, dried, and crystallised from ethyl acetate, when it separated in pearly leaflets, melting at 68°, and was identified as hentriacontane. (Found, C = 84‘9; H = 14'9. Calc., C = 85'3; II = 14‘7 per cent.) Isolation of a Pliytosterol, C27H4f)0. The alcoholic liquid from which the above-described mixture of incarnatyl alcohol and hentriacontane had separated was concen¬ trated to about half its volume, when a further quantity of solid was deposited. This was collected, and found to consist of a mixture of hentriacontane and a phytosterol. On concentrating the mother liquors, an additional quantity of substance was obtained, which, after crystallisation from a mixture of ethyl acetate and dilute alcohol, separated in fine, glistening needles, melting at 135—136°: 0-4994 lost 0-0248 H20 at 110°. H20 = 4-9. 9-1056 * gave 0-3244 C02 and 0-1160 H20. C = 83”8; H = 12’2. C27H4ri0,II20 requires H20 = 45 per cent. C27H4f)0 requires C = 839; H = 119 per cent. This substance is thus seen to agree in composition with a phytosterol, and it yielded the colour reaction of this class of compounds. A determination of its optical rotatory power gave the following result: 0*2478 of anhydrous substance, made up to 20 c.c. with chloro¬ form, gave aD —1°2/ in a 2-dcm. tube, whence [a]D —41 7°. The acetyl derivative, when crystallised from acetic anhydride, separated in needles, melting at 124—125°. 01860, made up to 20 c.c. with chloroform, gave aD — in a 2-dcm. tube, whence [a]D — 439°. The final mother liquors, after the separation of the above- described phytosterol, were evaporated until all the alcohol was removed. The residue consisted of a semi-solid mass, which was dis¬ tilled under diminished pressure, a.nd then amounted to 94 grams. It was found to consist chiefly of liquid hydrocarbons, with some oxygenated substances, but from it there could be isolated only a very small quantity of an alcohol, which crystallised in leaflets, melting at 88°. * Anhydrous substanee.