The bacteriological examination of the renal secretion in certain of the zymotic diseases, with subsidiary differential experiments / by C. J. Lewis.

  • Lewis C. J.
Date:
1901
Catalogue details

Licence: In copyright

Credit: The bacteriological examination of the renal secretion in certain of the zymotic diseases, with subsidiary differential experiments / by C. J. Lewis. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    In glucose jelly at 24 ' C.—Gas is formed after several days, less often in twenty-four hours. In milk at 37° C—Coagulation with acid formation in forty-eight hours. On blood serum.—As on agar. In litmus broth.—Acid is produced in twenty-four hours. Streptococcus 1 was present in the urine in two cases, and in both it was associated with albuminuria. It appeared late in the disease, namely, in the fourth and tenth weeks respectively, and was not present later when the albuminuria had ceased. Streptococcus 2.—Morphology and cultural characters.—In 1 per cent, peptone broth at 37° C.—After twenty-four hours there is slight general turbidity throughout the tube, but the chief growth is in mass at the bottom, whence it can be shaken up in viscid threads and fiocculent masses. After a few days there is marked general turbidity, with large deposit at the bottom of the tube resembling pus in urine. In ten days the broth is viscous and mucoid. With a power of 800 diameters, and examined in hanging-drop, the elements are recognised as cocci, either single or in pairs, but mainly in chains of short length, consisting of six to ten elements, or it may be up to twenty elements in each. The elements are of small size. Indol reaction.—Broth cultures give a positive indol reaction in a week or ten days, but in casein-peptone solution the reaction is positive on the fourth day. On agar at 37° C.—A flat, wliite-grey growth (not differentiated into separate colonies) with crenated edges and a moist glistening surface. There is some growth in mass in the expressed fluid at the bottom of the agar tube. In gelatin plate.—In twenty-four hours the colonies liaye a pearly- grey opacity and a smooth, glistening, raised surface. If kept at room temperature for a fortnight, the plate shows both superficial and deep colonies. The superficial are white, raised, with very slight central depression, and resemble drops of wax. They have a glistening appear- ance, and an outline roughly circular. The larger and older colonies are sometimes concentrically ringed. The deep colonies are small, and are oval or circular in shape. In stab jelly, growth occurs along the whole stab as a white or white- grey sheath, and towards the bottom of the stab isolated, rounded colonies are formed. There is no liquefaction, and there may be some gas production along the stab. On the surface of the jelly a white growth extends well over the surface, and has a more or less irregularly crenated margin. In glucose jelly at 24° C. gas is produced generally within twenty- four hours. In litmus broth at 37° C. acid is produced rapidly, the colour chang- ing in twenty-four hours. In milk at 39u C.—Coagulation and separation of whey in forty- eight hours, often in twenty-four hours. The whey is colourless and acid in reaction. On blood, serum the appearance resembles that on agar, but the growth is, if anything, more white and plentiful.