Report of experiments and observations on the vitality of the bacillus of typhoid fever and of sewage microbes in oysters and other shellfish / by E. Klein.

  • Klein, E. (Edward), 1844-1925.
Date:
[1905]
Catalogue details

Licence: Public Domain Mark

Credit: Report of experiments and observations on the vitality of the bacillus of typhoid fever and of sewage microbes in oysters and other shellfish / by E. Klein. Source: Wellcome Collection.

    32/94 (page 28)
    that is, 0*3 c.c. of the oyster, showed, as near as could be counted, 6400 colonies, that is, about 40,000 B. typhosus for the whole fish. As regards the oysters kept in sea water in the tub, the tub and the sterile sea water were changed after one, two, three, five, six, and seven days; oysters of the wet lot were analysed one day after change, two, five, six, seven, and nine days ; of the dry lot we analysed after one day dry, two, three, five, six, seven, and nine days dry. One day after change, oyster 3 (wet)—total amount of fluid 3 c.c., c.c. for one Drigalski plate, contains 46 colonies of B. typhosus—this would amount to 46 x 30 = 1380 B. typhosus per whole oyster. One day dry, oyster 2—total amount of fluid 4 c.c., yq c.c. for one Drigalski plate, contains 1000 colonies of B. typhosus—this would amount to 1000 x 40 = 40,000 B. typhosus per whole oyster. Two days after change in sterile sea water, oyster 5 (wet) —total fluid 2 c.c.; two days, oyster 4 (dry)—total fluid 1 c.c.; of each oyster c.c. for one Drigalski plate. Total number of colonies of B. typhosus in Drigalski plate of oyster 5 were 22, that is, 22 x 20 = 440 B. typhosus per whole oyster; Drigalski plate of oyster 4 contained 377 colonies of B. typhosus—this amounts to 3770 B. typhosus for the whole oyster. Three days dry, oyster 6—total fluid 1 c.c.; c.c. was used for one Drigalski plate, which developed 70 colonies of B. typhosus—this amounts to 700 B. typhosus for the whole oyster. Five days after change in sterile sea water, oyster 7— total fluid a little under 2 * 8 c.c., of this made two Drigalski plates each with y^- c.c. Total number of colonies of B. typhosus in the two plates was six (two in one, four in the second plate)—this would amount to about 82 B. typhosus for the whole oyster. Five days dry, oyster 8—total amount 1*5 c.c.; y1^ c.c. produced in a Drigalski plate 10 colonies of B. typhosus— this would amount to 150 B. typhosus per whole oyster. Six days after change in sterile sea water, oyster 9—total